Isolation and Identification of a Strain of Lactococcus garvieae from Xizang Yaks
In order to clarify the pathogen causing mastitis in Xizang yaks,milk samples of yak with mastitis were collect for bacterial culture.A strain of bacteria isolated was named as XZ-1,and staining microscopy,and 16S rRNA gene amplification and comparison.The 16S rRNA gene of the most similar bacteria was download from GenBank.DNA Star software was used to analyze homology and genetic evolution.The results showed that the isolated strains of bacteria was positive,scattered or arranged in pairs or short chains of spherical bacteria by Gram-stained and microscopic examination.The 16S rRNA gene comparison showed that it had a similarity of 99.43%to 99.93%with Lactococcus garvieae in GenBank,and a simi-larity of 99.5%to 100%with the reference strain of Lactococcus garvieae.Genetic evolution analysis showed that the Xizang isolate strain XZ-1 formed a large branch with the reference strain of Lactococcus garvieae,and was closely related to the reference strains of Lactococcus garvieae SCH and MS4-2,forming a cluster.Studies have shown that this isolated strain is Lactococcus garvieae and is one of the pathogens causing mastitis,which provided a reference basis for future research on the pathogen of yak mastitis and Lactococcus garvieae.
TibetYakLactococcus garvieaeisolation and identification
NETL
NSTL
万方数据
维普
