CKAP2L在卵巢癌中的表达及临床意义
Expression and Clinical Significance of CKAP2L in Ovarian Cancer
柴莹 1倪妍 1赵媛 1王晓 2杨晓洁1
作者信息
- 1. 太原市中心医院妇科,太原 030009
- 2. 太原市中心医院病理科,太原 030009
- 折叠
摘要
目的 本研究主要分析细胞骨架相关蛋白2样蛋白(cytoskeleton associated protein 2 like protein,CKAP2L)在卵巢癌中的表达及临床意义.方法 选取2018年2月至2020年5月在太原市中心医院行卵巢癌根治术的卵巢癌患者87例,收集其卵巢癌组织及距癌灶边缘＞2 cm的癌旁组织.实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)法测定卵巢癌组织及癌旁组织中 CKAP2L 的 mRNA 表达,免疫组化法分析卵巢癌组织及癌旁组织中CKAP2L的蛋白表达.Kaplan-Meier法分析卵巢癌组织中CKAP2L表达与患者预后的关系.结果 CKAP2L在卵巢癌组织中mRNA表达显著高于癌旁组织(P＜0.05).卵巢癌组织中CKAP2L的蛋白阳性表达率显著高于癌旁组织(P＜0.05).卵巢癌组织中CKAP2L mRNA表达与患者肿瘤分化程度、淋巴结转移、FIGO分期、浸润深度有关(P＜0.05).Kaplan-Meier分析结果表明,卵巢癌患者3年总生存率为80.46％;CKAP2L低表达患者3年总生存率为显著高于CKAP2L高表达患者(Log-rankx2=8.316,P=0.004).患者3年无复发生存率为77.01％;CKAP2L低表达患者3年无复发生存率显著高于CKAP2L高表达患者(Log-rank x2=8.921,P=0.003).结论 CKAP2L在卵巢癌组织中高表达,与FIGO分期、淋巴结转移等有关,是卵巢癌患者死亡的影响因素.
Abstract
Objective To analyze the expression and clinical significance of cytoskeleton-associated protein 2-like protein(CKAP2L)in ovarian cancer.Methods From February 2018 to May 2020,87 ovarian cancer patients who underwent radical surgery for ovarian cancer at Taiyuan Central Hospital were selected,and their ovarian cancer tissue and paracancer tissue＞2 cm from the edge of the cancer lesion were collected.The quantitative real-time polymerase chain reaction(qRT-PCR)method was applied to determine the mRNA expression of CKAP2L in ovarian cancer tissue and paracancer tissues,immunohistochemical methods were applied to analyze the protein expression of CKAP2L in ovarian cancer tissue and paracancer tissues.Kaplan-Meier method was applied to analyze the relationship between CKAP2L expression and patient prognosis in ovarian cancer tissue.Results The mRNA expression of CKAP2L in ovarian cancer tissue was higher than that in paracancer tissues(P＜0.05).The positive expression rate of CKAP2L protein in ovarian cancer tissue was higher than that in paracancer tissues(P＜0.05).The expression of CKAP2L mRNA in ovarian cancer tissue was related to the degree of tumor differentiation,lymph node metastasis,FIGO staging,and depth of invasion in patients(P＜0.05).Kaplan-Meier analysis results indicated that the 3-year overall survival rate of ovarian cancer patients was 80.46％;the 3-year overall survival rate of CKAP2L low expression patients was higher than that of CKAP2L high expression patients(Log-rank x2=8.316,P=0.004).The 3-year recurrence-free survival rate of patients was 77.01％;the 3-year recurrence-free survival rate of CKAP2L low expression patients was higher than that of CKAP2L high expression patients(Log-rank x=8.921,P=0.003).Conclusions CKAP2L is highly expressed in ovarian cancer tissue and is related to FIGO staging,lymph node metastasis,etc.It is a risk factor for death in ovarian cancer patients.
关键词
细胞骨架相关蛋白2样蛋白/卵巢癌/临床意义Key words
cytoskeleton-associated protein 2-like protein/ovarian cancer/clinical significance引用本文复制引用
出版年
2024
NETL
NSTL
万方数据