Detection and imaging of hydrogen sulfide and biological thiols in colorectal cancer WS480 cells
Objective To design and synthesize a novel fluorescent probe for the detection and imaging of biothiols and hydrogen sulfide in colorectal cancer cells,with the expectation of providing new tools and methods for the early diag-nosis of colorectal cancer.Methods The designed probe,WZL-1,was synthesized from commercially available and in-expensive raw materials through a four-step reaction.Its optical properties,cytotoxicity,and fluorescence imaging in liv-ing cells were evaluated in detail.The probe's fluorescence characteristics,sensitivity to biothiols and hydrogen sulfide,and its suitability for live cell imaging were analyzed.Results Under the physiological conditions,the probe WZL-1 ex-hibited significantly enhanced emission intensities at 630 nm for thiols cysteine,homocysteine,glutathione,and hydrogen sulfide.The probe demonstrated excellent sensitivity,and rapidly and accurately detected target molecules within a short period of time of 30 s,with fluorescence intensity maintained for at least 50 min.The WZL-1 fluorescence probe had a wide linear range,with a detection limit as low as 554 nm for GSH.The cell survival rate was more than 90%over the concentration range of 0-30 μmol/L.Conclusions The fluorescent probe WZL-1 can specifically react with bio thiols and hydrogen sulfide in colorectal cancer WS480 cells,emitting red fluorescence signals,while other substances do not react.It can be used for real-time monitoring and imaging of various biothiols and hydrogen sulfide in cells.
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