Protective effect of Xuebijing injection on lung injury in sepsis mice by regulating the HMGB1/TLR4/NF-κB pathway
Objective To investigate the protective effect of Xuebijing injection on lung injury in septic mice by reg-ulating the high mobility group protein box 1(HMGB1)/Toll-like receptor 4(TLR4)/nuclear factor-κB(NF-κB)pathway.Methods Male C57BL/6 mice were randomly divided into control,model,low,medium and high dose of Xuebijing groups,negative control(NC)group,NC+model group,NC+high-dose of Xuebijing group,and HMGB1+high-dose of Xuebijing group.A sepsis-induced lung injury model was established using cecal perforation.Before mod-eling,the mice were intravenously injected with NC lentivirus or HMGB1 lentivirus via the tail vein.On the day of mod-eling,the mice were intraperitoneally injected with Xuebijing injection(5,10 and 15 mL/kg)twice a day for three con-secutive days.Samples were collected and detected 24 h after the last administration.The pathological changes of lung tissue,wet weight(W)/dry weight(D),tumor necrosis factor-α(TNF-α),interleukin-1 β(IL-1β),interleukin-6(IL-6),malondialdehyde(MDA)and superoxide dismutase(SOD)contents,the expression levels of Cleaved caspase-3,HMGB1,TLR4 and NF-κB were compared among all groups.Results Pathological changes of lung injury were ob-served in the model group,with increases in the levels of W/D,TNF-α,IL-1β,IL-6,MDA,and expression of Cleaved caspase-3,HMGB1,TLR4,NF-κB,and decreased SOD content compared with the control group(P＜0.05).In Xuebijing treatment groups,pathological changes in lung injury were alleviated,with lower levels of W/D,TNF-α,IL-1β,IL-6,MDA and expression of Cleaved caspase-3,HMGB1,TLR4,NF-κB,and higher SOD content than the model group(P＜0.05).The HMGB1+high-dose of Xuebijing group showed aggravated lung injury pathology,with higher levels of W/D,TNF-α,IL-1β,IL-6 and MDA,and expression of Cleaved caspase-3,HMGB1,TLR4 and NF-κB,and lower SOD content than the NC+high-dose of Xuebijing group(P＜0.05).Conclusions Xuebijing injection has protective effect on lung injury in septic mice and alleviates inflammation,oxidative stress and apoptosis.The molec-ular mechanism related to this effect is the inhibition of HMGB1/TLR4/NF-κB pathway.
sepsislung injuryXuebijing injectionhigh mobility group box 1signaling pathway
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