Objective To evaluate the changes in skin lesions and in vivo microenvironment of an animal model of blood heat psoriasis using Rhizoma Zingiberis and Cinnamomum Cassia combined with imiquimod cream application.Methods 48 8-week-old healthy BALB/c mice(half male and half female)were randomly divided into a normal control group and a psoriasis model group.Starting from the 1st day,the model group was given 0.9 g/mL of Rhizoma Zingiberis and Cinnamomum Cassia decoction by gavage at a dose of 0.1 mL/10 g·d,while the control group was given 0.1 mL/10 g·d of physiological saline by gavage for 12 days.Starting from the 6th day of gastric lavage,the model group applied 42 mg of imiquimod cream on the back,while the control group applied an equal amount of vaseline cream on the back.The skin lesions were observed daily for 7 days.On the 13th day,after anesthesia with a small animal anesthesia machine,blood was collected from two groups of mice,and ELISA was used to detect the levels of serum in both groups of tumor necrosis factor-α(TNF-α),interferon-γ(IFN-γ),interleukin-21(IL-21)and interleukin-17(IL-17).Skin samples were taken from the back of two groups of mice for pathological tissue sectioning to observe the condition of skin lesions.Results The skin epidermis at the application site of the model group mice thickened,with uneven surface and scattered erythema.Under the microscope,it showed incomplete keratinization of the epidermis,thickening of the spinous layer,and increased sebaceous glands;The levels of TNF-α,IFN-γ,IL-21 and IL-17 in serum both increased,and compared with the control group,the difference was statistically significant(P<0.05).Conclusion BALB/c mice were orally administered a decoction of Rhizoma Zingiberis and Cinnamomum Cassia for 12 days,and from the 6th day onwards,imiquimod cream was added to the back for 7 consecutive days.A blood heat psoriasis animal model was successfully constructed,and the changes in skin lesions and internal microenvironment were consistent with the characteristics of psoriasis patients.
PsoriasisBALB/c miceAnimal model of psoriasisRhizoma ZingiberisCinnamomum cassiaImiquimod cream
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