Objective To investigate the role of wild-type P53-induced phosphatase1(WIP1)and its correlation with myosin light chain kinase 3(MYLK3)in oral squamous cell carcinoma(OSCC).Methods The expression of WIP1 and MYLK3 in 65 pairs of OSCC and adjacent normal oral mucosa tissues was detected by immunohistochemical ABC method.The expression of WIP1 and MYLK3 in OSCC was verified by the Cancer Genome Atlas(TCGA)database.The relationship between the expression of WIP1 and the clinicopathological parameters such as age,gender,tumor differentiation and lymph node metastasis and the correlation between WIP1 expression and MYLK3 were analyzed.Results The positive expression rate of WIP1 in OSCC was significantly higher than that in adjacent normal oral mucosa(47.7%vs 24.6%,P<0.05).The TCGA database analysis of 32 pairs of OSCC and adjacent normal oral mucosa tissues showed that the positive expression rate of WIP1 and MYLK3 in OSCC were significantly higher than those in adjacent normal oral mucosa tissues.The positive expression rate of WIP1 in the poorly differentiated group was significantly higher than that in the well differentiated group(86.7%vs 36.0%,P<0.05).The positive expression rate of WIP1 in the group with lymph node metastasis was significantly higher than that in the group without lymph node metastasis(90.0%vs 40.0%,P<0.05).There was no correlation between WIP1 and gender,age,tumor location and tumor size(P>0.05);There was a positive correlation between the expression of WIP1 and MYLK3 in OSCC(r=0.531,P<0.001).Conclusion WIP1 may play an important role in the tumorigenesis,development,differentiation and metastasis of OSCC,and may be a new potential biomarker for the occurrence,development and lymph node metastasis of OSCC.The mechanism of WIP1 may be related to the positive synergistic effect of MYLK3 in OSCC.
NETL
NSTL
万方数据
