Objective To investigate the effects of milk exosomes in immune system of neonatal mices.Methods Separation and identification of milk exosomes using high-speed centrifugation,and detection of immunoglobulin G(IgG),immunoglobulin A(IgA),immunoglobulin M(IgM),transforming growth factor-β(TGF-β),and epidermal growth factor(EGF)concentrations in milk exosomes using enzyme-linked immunosorbent assay(ELISA);36 7-day-old KM newborn mice were randomly divided into a breast milk group,a formula milk group,and a formula milk+milk exosomes group,with 12 mice in each group,and fed continuously for 14 days.Six KM mice were euthanized on the 7th and 14th day respectively,and blood samples were collected for routine blood tests.ELISA was used to detect the concentrations of IgG,IgA,IgM,TGF-β,and EGF in the serum.Results ELISA results showed that milk exosomes contained IgG,IgA,IgM,TGF-β and EGF.Compared with the breast milk group and the milk+milk exosome group,the concentrations of serum IgG,IgA and IgM in the milk group were significantly decreased on day 7 and day 14(P<0.05).Compared with milk group,serum IgG,IgA and IgM concentrations in milk+milk exosome group were significantly increased(P<0.05).The contents of TGF-β and EGF were not significantly changed.The blood routine results showed hemoglobin(HGB)and red blood cell(RBC)in milk group were significantly decreased(P<0.05),mean corpuscular hemoglobin concentration(MCHC)and platelet(PLT)were significantly increased(P<0.05),LYM in milk+exosome group was significantly decreased(P<0.05),GRA was significantly increased(P<0.05).Conclusion Milk exosomes contain IgG,IgA,IgM,TGF-β,and EGF,and can promote the expression of immune factors in infant mice fed with milk powder,enhancing the immune ability of newborn mice.
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