Objective Exploring the value of next-generation sequencing technology in the diagnosis and treatment of lung infections in immunosuppressed host. Methods 100 patients with lung infections in immunosuppressed hosts hospitalized in the First People's Hospital of Zhaoqing from 1 July 2022 to 30 June 2023 were selected and grouped into groups of 50 cases each using the single and double number method. Sputum samples and alveolar lavage fluid were taken from the two groups of patients,and the pathogens were detected using microbiological culture-dependent conventional assays and multiplex PCR-based targeted next-generation sequencing (tg-NGS),respectively,and the appropriate treatments were administered based on the results of the assays. The sensitivity of conventional and tg-NGS tests for detecting lung infections was analysed statistically,and the relationship between drug administration based on the results of conventional and tg-NGS tests and the patients' temperature recovery time and hospital stay was analysed. Results The detection rates of bacterial and fungal pathogens in both the observation and control groups were high;the detection rates of viral pathogens in the observation group were significantly higher than those in the control group (P<0.05),and the detection rate of viral pathogens in the control group was 0;most of the pathogens detected in the control group were single pathogens,and only two cases were detected as double pathogens;whereas,the detection rates of two or more pathogens in the observation group were significantly higher than those in the control group (P<0.05). The detection rate of two or more pathogens in the observation group was significantly higher than that in the control group (P<0.05). Compared with the traditional test,tg-NGS significantly increased the detection rate of lung infection pathogens and improved the sensitivity of lung infection detection (P<0.001);compared with the traditional test treatment,tg-NGS significantly reduced the levels of inflammatory factors such as serum C-reactive protein (CRP),interleukin-6 (IL-6) and tumour necrosis procalcitonin (PCT) (P<0.001);and significantly shortened the patients' temperature recovery time and hospitalization time (P<0.001). Conclusion The tg-NGS test improves the detection of pathogenic bacteria in lung infections and increases the sensitivity of lung infection detection. Precise treatment based on tg-NGS results accelerates patient healing,avoids drug abuse,and shortens hospitalization time.
关键词
二代测序/靶向二代测序/免疫抑制/肺部感染/精准医学
Key words
Next-generation sequencing/Tg-NGS/Immunosuppression/Lung infection/Precision medicine
万方数据