桔梗皂苷D对人结肠癌细胞中程序性细胞死亡配体-1表达和血管生成的影响
Effects of platycodin D on programmed death ligand 1 expression and angiogenesis in human colon cancer cells
李程程 1李光亿 1邰屹 1刘欣哲 1曹晟 1马娟1
作者信息
- 1. 长白山天然药物教育部重点实验室 延边大学药学院 分子药物研究中心,吉林 延吉 133002
- 折叠
摘要
[目的]探讨桔梗皂苷D(PLD)对人结肠癌细胞的程序性细胞死亡配体-1(PD-L1)表达、集落形成、伤痕愈合及内皮细胞血管生成的影响.[方法]采用MTT法检测PLD对人结肠癌细胞HCT116 细胞增殖的影响,蛋白免疫印迹法检测PLD对HCT116 细胞中PD-L1 蛋白表达的影响,细胞集落法观察PLD对HCT116 细胞增殖能力的影响,划痕实验法观察PLD对细胞迁移速率的影响,小管形成实验法观察PLD对内皮细胞血管生成的影响.[结果]PLD可抑制肿瘤细胞增殖(P＜0.001),以浓度和时间依赖性地抑制HCT116细胞中PD-L1蛋白的表达,抑制HCT116 细胞集落的形成、迁移和内皮细胞血管的生成.[结论]PLD可抑制结肠癌HCT116 细胞中的PD-L1表达,抑制HCT116 细胞的增殖、迁移及血管生成.
Abstract
OBJECTIVE To explore the effect of platycodin D(PLD)on programmed death ligand 1(PD-L1)expression,colony formation,wound healing and angiogenesis of endothelial cells in human colon cancer cells.METHODS MTT assay was used to detect the effect of PLD on the proliferation of human colon cancer cells HCT116.Western blot assay was used to detect the effect of PLD on the expression of PD-L1 protein in HCT116 cells.Cell colony formation experiment was used to observe the effect of PLD on the proliferation ability of HCT116 cells.Scratch assay was used to observe the effect of PLD on cell migration rate.Tubule formation assay was used to observe the effect of PLD on the angiogenesis of endothelial cells.RESULTS PLD could inhibit the proliferation of tumor cells(P＜0.001),and inhibit the expression of PD-L1 protein in HCT116 cells in a concentration and time dependent manner.PLD could inhibit the cell colony formation and migration of HCT116 cells and angiogenesis of endothelial cells.CONCLUSION PLD can inhibit the expression of PD-L1 in human colon cancer HCT116 cells,and inhibit the proliferation,migration of HCT116 cells and angiogenesis.
关键词
桔梗皂苷D/结肠癌/PD-L1/血管生成Key words
platycodin D/colon cancer/PD-L1/angiogenesis引用本文复制引用
基金项目
吉林省大学生创新创业训练计划(2023YDCXCY058)
国家自然科学基金(81760657)
出版年
2024
NETL
NSTL
万方数据