首页|网络药理学方法研究鹅不食草治疗胃癌的作用机制

网络药理学方法研究鹅不食草治疗胃癌的作用机制

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[目的]运用网络药理学方法探讨鹅不食草治疗胃癌的作用机制.[方法]基于中药系统药理学数据库中鹅不食草的相关研究,分析预测其主要活性成分.检索SwissTargetPrediction和SEA数据库获得鹅不食草相关靶点基因;检索中药系统药理数据库(TCMSP)获得胃癌靶点基因.在Venny2.1.0 数据库中比对鹅不食草的主要活性成分与胃癌的相同靶点,利用Cytoscape3.9.1 软件构建药物-成分-靶点-疾病网络图,结合STRING数据库对共同靶点进行蛋白-蛋白相互作用分析并构建PPI网络,确定核心靶点;利用Metascape平台对核心靶点进行GO富集分析与KEGG信号通路富集分析,在微生信在线绘图平台中绘制柱状图及气泡图反映研究结果.[结果]探索TCMSP数据库共获得化学成分 108 个,筛选后确定 17 个有效化学成分及 757 个成分靶点,与 225 个胃癌相关靶点取交集,得到 36 个共同靶点,涉及 18 条信号通路.[结论]Ras、Rap1、PI3K-Akt及MAPK 4 个信号通路上的PDGFRB、PLAU、PLA2G1B及CDC25B基因与鹅不食草治疗胃癌的分子机制具有较强的联系.
The mechanism of Herba Centipedae in the treatment of gastric cancer studied by network pharmacology
OBJECTIVE To explore the mechanism of Herba Centipedae in the treatment of gastric cancer studied by network pharmacology methods.METHODS Based on the related research of Herba Centipedae in the database of Traditional Chinese Medicine Systems Pharmacology(TCMSP),the main active ingredients of Herba Centipedae were analyzed and predicted.SwissTargetPrediction and the SEA database were used to obtain the target genes related to Herba Centipedae,and gastric cancer target genes were retrieved from the GEO database.In the Venny 2.1.0 database,the same targets of the main active ingredients of Herba Centipedae and gastric cancer were compared.The drug-ingredient-target-disease network diagram was constructed using Cytoscape 3.9.1 software,and the protein-protein interaction analysis was performed on the common targets combined with the STRING database,and PPI network was constructed to determine the core targets.Metascape platform was used to conduct GO enrichment analysis and KEGG signal pathway enrichment analysis for the core targets,and column charts and bubble charts were drawn on bioinformatics plotting platform to reflect the research results.RESULTS A total of 108 chemical ingredients were obtained by exploring the TCMSP database,and 17 effective chemical ingredients and 757 ingredient targets were identified after screening,and 36 common targets were obtained by intersecting with 225 gastric cancer related targets,involving 18 signaling pathways.CONCLUSION PDGFRB,PLAU,PLA2G1B,and CDC25B genes in the Ras,Rap1,PI3K-Akt and MAPK signaling pathways have strong associations with the molecular mechanisms of Herba Centipedae in the treatment of gastric cancer.

Herba Centipedaegastrict cancernetwork pharmacologyintegration of traditional Chinese and western medicine

史家萍、李晓飞

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锦州医科大学,辽宁 锦州 121000

鹅不食草 胃癌 网络药理学 中西医结合

2024

延边大学医学学报
延边大学

延边大学医学学报

影响因子:0.308
ISSN:1000-1824
年,卷(期):2024.47(1)
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