Effects of combined immunohistochemical staining of P16 and Ki-67 on the grading diagnosis of cervical intraepithelial neoplasia
OBJECTVE To explore and analyze the effect of combined immunohistochemical staining of p16 and Ki-67 on the grading diagnosis of cervical intraepithelial neoplasia(CIN).METHODS The clinical data of 60 patients with abnormal results of cervical liquid-based thin layer cytology(TCT)admitted to our hospital from March 2019 to November 2021 were retrospectively analyzed.All patients were divided into chronic cervicitis group(n=19),CIN 1 group(n=15),CINⅡ group(n=12),CINⅢ group(n=10)and cervical cancer group(n=4),all patients underwent cervical colposcopy biopsy,human papillomavirus(HPV)typing,and semi-quantitative immunohistochemical staining with P16 and Ki-67.score to judge the degree of cervical lesions in patients.RESULTS With the increase of the degree of cervical CIN lesions,the positive degree of P16 expression and Ki-67 expression were higher,and the difference was statistically significant(P＜0.05).In the chronic cervicitis group,P16 and Ki-67 immunohistochemistry combined with semi-quantitative scores were completely consistent with the CIN grading results of HE staining.The consistent rate of CIN Ⅰ,CIN Ⅱ and CINⅢ groups was 94.59％,and the difference was statistically significant(P＜0.05).Positive expression of P16 was positively correlated with HPV infection(P＜0.05).HPV-16,HPV-18,HPV-35,HPV-39 and HPV-51 showed significant differences in the positive and invisible expression of P16(P＜0.05).CONCLUSIONS The CIN grade of cervical intraepithelial neoplasia has a significant positive correlation with the expression of P16 and Ki-67,and P16 is closely related to HPV infection in patients.
cervical intraepithelial neoplasia gradingP16Ki-67immunohistochemical stainingcervical human papillomavirus infection
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