OBJECTIVE To determine the content of total flavonoids in Ulmus davidiana var.japonica by ultraviolet-visible spectroscopy(UV-Vis)after optimizing the extraction technology of total flavonoids from Ulmus davidiana var.japonica.METHODS The optimal extraction technology of Ulmus davidiana var.japonica for total flavonoids extraction rate was determined by orthogonal test based on temperature,ethanol volume fraction and time.The total flavonoid content in Ulmus davidiana var.j aponica was detected by UV-Vis.RESULTS The optimal extraction conditions for total flavonoids in Ulmus davidiana var.j aponica were 50%ethanol,a temperature of 75℃,and a duration of 100 minutes.Under these conditions,the extraction rate of total flavonoids was 9.69%.CONCLUSION Ethanol reflux extraction method is cheap and easy to implement.The orthogonal experimental design method optimizes the extraction conditions for total flavonoids in Ulmus davidiana var.j aponica,which is simple,feasible,and highly reproducible,and provides a valuable reference for research on total flavonoids in Ulmus davidiana var.j aponica.
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