Determination of mercury content in trace urine by atomic fluorescence spectroscopy and its application
Objective:To investigate the determination of mercury content in trace urine by atomic fluorescence spectrometry and apply it to the determination of mercury content in urine of certain college students.Methods:An atomic fluorescence spectrometry method for the determination of mercury content in microurine was established and evaluated by linear range,correlation coefficient,detection limit,precision and recovery rate.The method was applied to the determination of mercury content in microurine of certain college students.Results:The methodological evaluation results showed that the linear range for the determination of mercury content in trace urine by atomic fluorescence spectrometry was 0.00-1.00 μ g/L,with a correlation coefficient of 0.9995,a detection limit of 0.005 μ g/L,a sample spiked recovery rate of 92.55%-97.48%,and a relative standard deviation of 0.22%-0.25%.The results of urine testing on college students showed that the distribution range of mercury content was 0.078-9.154 μ g/L,with an average value of 3.448 μg/L.The average level of mercury content in urine between male and female college students was similar,and there was no statistically significant difference between genders(P<0.05).Conclusion:The method established under the experimental conditions is easy to operate,highly sensitive,and has a low detection limit,which can provide a reference for the detection of mercury content in microbiological samples in the future.
万方数据
维普
