Quantitative polymerase chain reaction(qPCR),referred to as fluorescent quantitative PCR,plays an important role in the molecular diagnosis of the clinical laboratory.However,undergraduate interns major in clinical laboratory have a relatively weak grasp of qPCR technology and limited internship duration.In order to ensure teaching quality and improve the professional knowledge and practical ability of interns,this paper summarized and discussed the teaching reform of qPCR technology based on the current practice status and teaching experience.The results showed that the interns'learning interest and knowledge mastery were obviously improved,and the teaching effect was good by clarifying and detailing the teaching objectives,carrying out the teaching in various forms,improving the assessment system and other measures.
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