宜宾学院学报2024,Vol.24Issue(6) :97-101.DOI:10.19504/j.cnki.issn1671-5365.2024.06.17

冬小麦根际细菌多样性及其促生活性

Diversity of Rhizosphere Bacteria of Winter Wheat and Their Plant-promoting Activities

张永光 李陈红 曾凡江
宜宾学院学报2024,Vol.24Issue(6) :97-101.DOI:10.19504/j.cnki.issn1671-5365.2024.06.17

冬小麦根际细菌多样性及其促生活性

Diversity of Rhizosphere Bacteria of Winter Wheat and Their Plant-promoting Activities

张永光 1李陈红 1曾凡江2
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作者信息

  • 1. 宜宾学院农林与食品工程学部,四川宜宾 644000
  • 2. 中国科学院新疆生态与地理研究所,新疆乌鲁木齐 830011
  • 折叠

摘要

以分离自新疆冬小麦根际的36株细菌为研究对象,通过16SrRNA基因序列测定、系统进化分析研究其多样性,并检测其溶磷和固氮活性.结果发现:(1)36株菌分布于3门5纲11目14科14属,其中a-变形菌门和放线菌纲为优势菌门(各占总菌株数的47.2%),红球菌为最主要的优势菌属(占总菌株数的36.1%),并有一个潜在的新物种;(2)以磷酸钙为底物,定性检测表明待测菌株中50.0%具有溶磷活性,定量测定的5株优选菌中菌株57溶磷活性最高(11.5 mg/L);(3)66.7%菌株具有固氮活性,其中45.8%具有较强固氮活性.

Abstract

36 strains of rhizosphere bacteria isolated from the winter wheat of Xinjiang were studied by 16S rRNA gene se-quence determination and phylogenetic analysis,and their phosphorus-solublization and nitrogen-fixing activities were tested.The results show that 36 strains are distributed in 3 phyla,5 classes,11 orders,14 families and 14 genera,among which Proteobacte-ria and Actinomycetota are the dominant phyla(47.2%and 47.2%of the total strains respectively),Rhodococcus is the major genus(36.1%of the total strains),and there is a potential new species;using calcium phosphate as substrate,the qualitative test shows that 50.0%of the tested strains have phosphorus-solubilizing activity;the phosphorus-solubilizing activities of strain 57 is the highest among the 5 strains tested quantitatively(11.5 mg/L);66.7%of the strains tested had nitrogen-fixing activity,and 45.8%of them had strong nitrogen-fixing activity.The results show that the diversity of rhizosphere bacteria of the winter wheat in Xinjiang is very rich,most of them have phosphorus-solubilizing or nitrogen-fixing activities.

关键词

冬小麦/根际细菌/多样性/促生活性

Key words

winter wheat/rhizosphere bacteria/diversity/growth-promoting activities

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基金项目

宜宾学院科学研究基金项目(2020YY01)

中国科学院"西部之光"基金项目(2017-XBQNXZ-A-003)

宜宾学院博士启动基金项目(2020QH04)

出版年

2024
宜宾学院学报
宜宾学院

宜宾学院学报

CHSSCD
影响因子:0.185
ISSN:1671-5365
参考文献量4
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