Three biocontrol strains with strong antagonistic effect against Fusarium fusarium were screened from the soil around the root of Zanthoxylum in Longnan,and identified as Bacillus subtilis W-5,Bacillus Bellescens T-1 and Bacillus Bellescens W-1 by morphological and molecular biological methods.After the fermentation conditions of 3 kinds of antagonistic bacteria were deter-mined,the composite microbial biocontrol agent was developed with the mixture of 3 kinds of antagonistic fermentation liquid.The best carrier of the composite bactericide is diatomite,and the adsorption capacity is 2.5 mL/g.The best dispersant is sodium ligno-sulfonate,the addition amount is 6％;The best UV protective agent is sodium alginate,the addition amount is 8％.The results show that the antibacterial activity of the compound bacteriological agent is 83.3％,79.3％,74.7％,89％and 83.7％,respectively,against Fusarium dermatitis,Fusarium syphilitica,Fusarium piniculata,Fusarium graminea and anthracnose of walnut.When diluted 2000 times,the inhibition rate of mycelium of pathogenic bacteria of Zanthoxylum root rot is over 95％.Its pH value is 6.75,water con-tent is 0.25％,effective viable bacteria number 3.17×108 CFU/g,shelflife of 0.8～1 years,up to GB 20287-2006 standard.
prickly ashroot rotmicrobial agentbiological control
维普
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