In order to improve the planting technology of high quality and yield of mid-season rice-ratooning rice in the rice crop-ping regions with less than two seasons of temperature and light resources,two kinds of organic fertilizers,microbial fertilizer and earthworm manure,and various substitution ratios were utilized to investigate the impact of organic fertilizer substitution on the yield and quality of mid-season rice-ratooning rice.The study shows that the appropriate substitution ratio of organic fertilizer sig-nificantly enhances the yield of first-season rice and ratooning rice.The highest yield is achieved when the substitution ratio of mi-crobial fertilizer and earthworm manure is 30%.In terms of quality,the head rice rate,total starch content,gel consistency,and taste value of first-season rice and ratooning rice increase with the higher substitution ratio of two organic fertilizers.Conversely,the gelatinization temperature and amylose content decrease as the substitution ratio of the two organic fertilizers increases.In addi-tion to the processing quality and protein content,the overall quality of rice is optimal when the replacement ratio is 45%.Although the yield of ratooning rice is lower than that of first-season rice,its quality is better than that of first-season rice.The effects of vari-ous organic fertilizer replacement treatments on yield and quality are consistent across the two growing seasons.In conclusion,the appropriate proportion of microbial fertilizer and earthworm manure instead of chemical fertilizer can achieve synergistic improve-ments in both yield and quality in mid-season rice-ratooning rice cultivation.