The sterile tissue culture seedlings were cultured with the C.ferruginea seed as raw material,tissue culture was studied by using the leaves of inoculant as explants.Suitable medium for each stage was screened by different plant hormone ratio and con-centration.The results show that the optimal medium for callus induction is MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+2,4-D 1.0 mg/L,the induction rate reached 100％after 30 days,browning rate is 20％,the callus is emerald-green,growing well.The optimal medium for callus proliferation is MS+6-BA 0.5 mg/L+NAA 0.5 mg/L+2,4-D 0.8 mg/L,the callus is emerald-green,growing well,there is no browning after 30 days.The optimal medium for cluster bud induction is MS+6-BA 1.5 mg/L+NAA 0.1 mg/L+TDZ 0.05 mg/L,and the callus differentiation rate was 100％after 60 days,the average number of multiple shoots is 5±0.54,and the average plant height is 5.5 cm,the occurrence of browning is a crucial prerequisite for the induction of the C.ferruginea callus differentiation.The optimal rooting medium is 1/2MS+IBA 1.5 mg/L+NAA 1.5 mg/L,the rooting rate is 98.89％after 60 days,the average number of main roots is 5,the mean root length is 4 cm,and has too many fibrous roots.PVP could markedly suppress the browning of C.ferruginea callus,and there is no browning in callus after 45 days,but it could inhibit callus expansion and growth.
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万方数据