Objective:To investigate the relevant risk factors and preventive measures for MHD patients with hepatitis virus infec-tion in hemodialysis room. Methods:risk factors for hepatitis virus infection in hemodialysis room were summarized and analyzed, and appropriate care measures were proposed for practical implementation. Results: after a detailed analysis of all aspects of blood dialysis room hospital infection, strict infection control measures were developed and implemented, which could effectively control the spread of hepatitis virus infection and improve the quality of life of patients, as well as protecting medical staff to the greatest extent.