作者信息
- 1. 湖南环境生物职业技术学院 421005
- 折叠
摘要
目的：：观察葛根异黄酮治疗去卵巢大鼠骨质疏松的效果。方法：选用健康3月龄SD雌性大鼠50只，随机分为5组：假手术组、模型对照组、低、中、高剂量葛根异黄酮组；假手术组切除卵巢旁脂肪组织，其余各组均切除双侧卵巢，建立去卵巢大鼠动物模型。按25、50、100 mg/kg 3个剂量葛根异黄酮分别给去卵巢大鼠灌胃6个月，第3、6个月测量体重，并于实验结束后处死大鼠，检测血清钙、磷、碱性磷酸酶水平和股骨中心、远心骨密度。结果：中、高剂量治疗组动物较模型对照组在第3、6个月体重均降低，在第6个月血清钙明显升高，血清磷和碱性磷酸酶水平明显降低。高剂量治疗组动物较模型对照组在第6个月股骨中心和远心骨密度均明显升高。结论：葛根异黄酮长期治疗可升高血清钙水平，降低血清磷和碱性磷酸酶水平，改善骨密度，具有抗骨质疏松的作用。
Abstract
Object:To observe the effect of Totalisoflavone on osteoporosistreatment inovariectomized rats.Methods:We randomly distributed 50 healthy and fe-male rats with 3-month of age into 5 groups:sham operation group, model control group and Total isoflavonespueraria groups with low, medium and high-dose ad-ministration. Thesham group was removed adipose tissuesaroundovarian, the others were all ovariectomizedrats.The ovariectomizedrats were model controls, or were intragastric administrated with Totalisoflavone at the dose of 25, 50 or 100 mg/kg. In the third and sixth month, weights of rats were observed. In the six month, the levels of Ca+, P and alkaline phosphatase (ALP) in serum, and the bone mineral density (BMD) in both center and far-end of femur were tested.Results:The weights of medium and high-dose groups were lower than the model controls in the third and sixth month. Compared with the model controls, the levels of Ca+in serum were increased, and the levels of P and ALP in serum were decreased in medium and high-dose groups in the sixth months. The BMDs in both center and far-end of femur were higher in high-dose group than the model controls in the sixth months. Conclusions: Treatment with Totalisoflavone in a long term could increase the level of Ca+in serum, decrease the level of P and ALP in serum, improve BMD, showing an effect of anti-osteoporosis.
关键词
Treatment with Totalisoflavone in a long term could increase the level of Ca+ in serum/decrease the level of P and ALP in serum/improve BMD/showing an effect of anti-osteoporosisKey words
Treatment with Totalisoflavone in a long term could increase the level of Ca+ in serum/decrease the level of P and ALP in serum/improve BMD/showing an effect of anti-osteoporosis引用本文复制引用
出版年
2015
NETL
NSTL
万方数据