Objective:To probe into the value of blood test, PCT and CRP in the early diagnosis of neonatal sepsis. Methods:The children patients with neonatal sepsis were selected in our hospital. And children patients with exclusion of infectious diseases during the same period were selected in our hospital. The changes of platelet count, neutrophil neutral rod ratio, PCT and CRP levels of al the children patients at different stages and control group were compared and analyzed. Results:Compared with the control group, the children patients with neonatal sepsis, the platelet count were decreased, granulocyte neutral rod ratio were improved, the PCT and CRP levels were increased significantly at infection, there were difference significant statistical y (P <0.05). The platelet count, granulocyte neutral rod ratio, PCT and CRP levels before and after infection were changed no significant statistical y. Conclusions: Combined with laboratory blood test, PCT and CRP testing, it could have very important guidance for early diagnosis and treatment.