水稻“光身”突变体glr3的遗传分析及基因定位
Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)
宋海冰 1汪斌 2陈壬杰 1郑小雅 1于世波 1兰涛1
作者信息
- 1. 福建农林大学，作物遗传育种与综合利用省部共建教育部重点实验室，福州 350002; 福建农林大学，福建省作物设计育种重点实验室，福州 350002
- 2. 福建农林大学，作物遗传育种与综合利用省部共建教育部重点实验室，福州 350002; 福建农林大学生命科学学院，福州 350002
- 折叠
摘要
在籼稻品种R401辐射诱变的M2群体中筛选到一个叶片表皮光滑突变体，在正常条件下，突变体叶片和颖壳光滑无毛。以毛叶粳稻品种Nipponbare和“光身”突变体作为亲本，构建了一个F2群体，通过调查该群体在正常种植条件下的表现，发现Nipponbare和“光身”突变体控制表皮毛性状的差异受单个主基因控制且“光身”为隐性，将该基因暂时命名为GLR3。利用该F2群体，采用BSA法将GLR3定位在第6染色体上，进一步对F2群体中417个典型的叶片光滑单株进行分子标记分析，将该基因定位在 InDel标记ID27101和ID27199之间，与两标记相距皆为0.1 cM，两标记的物理位置相距98 kb。
Abstract
We obtained a glabrous leaf and hull mutant from a population of radiation mutagenesis of an indica rice cultivar R401. The mutant produced smooth leaves and hairless glumes under normal growth conditions. An F2 population was developed from a cross between a japonica cultivar Nipponbare and the glabrous leaf and hull mutant. By investigating the performance of the F2 population, we found that the mutant phenotype was controlled by a single recessive gene, temporarily designated GLR3. Bulked segregant analysis (BSA) based on the F2 mapping population revealed that GLR3 is located on chromosome 6. By analyzing 417 typical glabrous leaf F2 plants using molecular markers, GLR3 was mapped to a 0.2 cM interval between InDel markers ID27101 and ID27199, and the physical distance between the two markers is 98 kb. Thus we have mapped the gene GLR3, and our work will provide basis for future mechanistic analysis of GLR3 function.
关键词
水稻/光身/遗传分析/基因定位Key words
rice/glabrous leaf and hull/genetic analysis/gene mapping引用本文复制引用
基金项目
国家自然科学基金(31671688)
福建省自然科学基金(2015J01094)
福建省教育厅科技计划项目(JK2012014)
出版年
2016
NETL
NSTL
维普
万方数据