Advances in the mechanisms and applications of RNA silencing in crop protection
RNA silencing(or RNA interference,RNAi)is a conserved mechanism for regulating gene expression in eukaryotes,which plays vital roles in plant development and response to biotic and abiotic stresses.The discovery of trans-kingdom RNAi and interspecies RNAi provides a theoretical basis for exploiting RNAi-based crop protection strategies.Here,we summarize the canonical RNAi mechanisms in plants and review representative studies associated with plant-pathogen interactions.Meanwhile,we also elaborate upon the principles of host-induced gene silencing,spray-induced gene silencing and microbe-induced gene silencing,and discuss their applications in crop protection,thereby providing help to establish novel RNAi-based crop protection strategies.
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