Ssu72 phosphatase deficiency leads to spindle crossing during the second meiotic division process
Ssu72 is a component of the yeast cleavage/polyadenylation factor(CPF)complex,which catalyzes the dephosphorylation of the C-terminal domain(CTD)of RNA polymerase Ⅱ at S5-P and S7-P.It has been shown that Ssu72 phosphatase is involved in regulating chromosome cohesion during mitosis.To further clarify whether Ssu72 phosphatase affects chromosome separation during meiotic division in Schizosaccharomyces pombe,we utilized green fluorescent protein(GFP)to label centromeres and red fluorescent protein to label microtubule protein Atb2.The entire meiotic chromosome separation process of ssu72∆ cells was observed in real-time under fluorescence microscope.It was found that two spindles of ssu72∆ cells crossed during the metaphase and anaphase of the second meiotic division,and this spindle crossing led to a new type of spore defect distribution pattern.The results of this study can provide important reference significance for studying the roles of phosphatase Ssu72 in higher organisms.
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