Effects of SvAPETALA1-5 gene on floral organ development in Senecio vulgaris
Asteraceae is a large class of eudicots with complex capitulum,and little is known regarding the molecular regulation mechanism of flower development.APETALA1(AP1)belongs to the MADS-box gene family and plays a key role in plant floral induction and floral organ development.In this study,the bioinformatics and tissue-specific expression of AP1 homologous gene SvAP1-5 in Senecio vulgaris were analyzed.Based on VIGS technology,SvAP1-5 gene silencing plants were created,and SvAP1-5 was overexpressed in Solanum nigrum.The results of bioinformatics analysis showed that SvAP1-5 gene had typical MADS-box and K-box structure,and contains FUL motif and paleoAP1 motif at the C-terminal.SvAP1-5 belongs to the euFUL branch of AP1 gene.qRT-PCR results showed that SvAP1-5 was expressed in bracts,petals and carpels,and was highly expressed in carpels.Compared with the control group,SvAP1-5 gene silencing resulted in irregular petal dehiscence,increased stigma division,and carpel dysplasia.The fruit development of SvAP1-5 overexpressing S.nigrum plants was abnormal,and the hyperplastic tissue similar to fruit appeared.In summary,SvAP1-5 gene may be involved in the development of petals and carpels and plays an important role during the development of S.vulgaris.
Senecio vulgarisSvAPETALA1-5flower developmentfunctional research
万方数据
维普
