首页|干旱胁迫下外源甜菜碱和脯氨酸对烤烟抗氧化代谢的影响

干旱胁迫下外源甜菜碱和脯氨酸对烤烟抗氧化代谢的影响

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为明确甜菜碱和脯氨酸在提高烤烟抗旱能力上的应用效果,采用盆栽试验,研究了外源喷施甜菜碱和脯氨酸对烤烟抗氧化代谢的影响.结果表明,干旱胁迫下,喷施甜菜碱和脯氨酸均能不同程度提高烤烟叶片叶绿素和可溶性蛋白质含量,降低丙二醛(MDA)含量,有效延缓植株衰老进程;能不同程度提高烤烟叶片超氧化物歧化酶(SOD)活性,同时促进渗透调节物质脯氨酸的积累,是提高烤烟抗旱能力的重要原因.脯氨酸和甜菜碱均可以作为烤烟干旱胁迫下重要的外源调节物质,在降低烤烟膜质过氧化程度方面,甜菜碱要优于脯氨酸,而二者同时施用能够有效提高烤烟过氧化氢酶(CAT)活性,降低MDA含量,其调控效应最佳.
Effects of Exogenous Glycine Betaine and Proline on Antioxidant Metabolism of Flue-cured Tobacco Under Drought Stress
In order to investigate the effects of glycine betaine and proline application on the resistance to drought, a pot culture experiment was carried out to study the effects of glycine betaine and proline spraying on the antioxidant metabolism of flue-cured tobacco under drought stress. The results showed that: l) Under drought stress, the contents of chlorophyll and soluble protein were increased, while MDA content was decreased to different extents by spraying glycine betaine or praline, which effectively delayed the consenescence of the plants. 2) Glycine betaine and proline spraying improved SOD activities to different extents and promoted proline accumulation in tobacco leaves, which were the important factors improving the drought resistance of flue-cured tobacco. 3) Either glycine betaine or proline could be used as exogenous regulating substance for flue-cured tobacco under drought stress. Glycine betaine was superior to proline in decreasing membrane lipid peroxidation in tobacco leaves, however, from the point of increasing CAT activity and decreasing MDA content, it would be more effective to apply the two together.

Flue-cured tobacco (Nicotiana tabacum L.)Glycine betaineProlineAntioxidant metabolism

梁太波、张景玲、田雷、王建伟、尹启生、蔡宪杰、刘阳、张仕祥

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中国烟草总公司郑州烟草研究院,45000

河南中烟工业有限责任公司安阳卷烟厂,455000

山东临沂烟草有限公司,276003

烤烟 甜菜碱 脯氨酸 抗氧化代谢

中国烟草总公司郑州烟草研究院院长基金山东省烟草公司科技重大专项

112009CA0220200902

2013

烟草科技
中国烟草总公司郑州烟草研究院

烟草科技

CSTPCD北大核心EI
影响因子:1.049
ISSN:1002-0861
年,卷(期):2013.(2)
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