首页|索氏提取-气相色谱-质谱法测定卷烟主流烟气中苯并[a]芘

索氏提取-气相色谱-质谱法测定卷烟主流烟气中苯并[a]芘

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  • 国家科技期刊平台
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  • NSTL
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  • 维普
为简化样品前处理操作步骤，减小分析误差，设计了一种带有柱层析净化功能的索氏提取装置，基于该装置建立了测定卷烟主流烟气中苯并[a]芘（B[a]P）释放量的索氏提取-气相色谱-质谱（GC/MS）方法，并采用该方法对肯塔基参比卷烟2R4F和14个不同类型的卷烟样品进行了测定。结果表明：①利用该装置可直接对捕集卷烟主流烟气总粒相物后的剑桥滤片进行提取、净化和浓缩，浓缩液用GC/MS在选择离子监测模式下进行测定。②方法的线性范围为5～500 ng/mL（R2=0.9995），B[a]P的加标回收率为95.4%～104.2%，平均相对标准偏差（RSD）2.32%，检测限0.12 ng/支。③2R4F参比卷烟和14个不同类型卷烟样品主流烟气中B[a]P的测定结果与采用GB/T 21130—2007方法的测定结果基本一致。该方法操作简便，灵敏度和准确度较高，适用于卷烟主流烟气中B[a]P的测定。
Determination of benzo[a]pyrene in mainstream cigarette smoke by Soxhlet extraction-gas chromatography-mass spectrometry
To simplify sample preparation and reduce analysis errors, a Soxhlet extraction system combined with column chromatography purification was established to determine the levels of benzo[a]pyrene (B[a]P) in mainstream cigarette smoke. Kentucky 2R4F reference cigarette and 14 cigarette samples were determined by the developed method. The results showed that: 1) The total particulate matter of mainstream cigarette smoke collected by a Cambridge filter pad was directly extracted, purified and concentrated by the improved Soxhlet extraction system. The extract was analyzed by gas chromatography-mass spectrometry under selected ion monitoring mode. 2) B[a]P displayed a good linearity (R2=0.999 5) in the range of 5 to 500 ng/mL, and the detection limit was 0.12 ng/cig(S/N=3). The developed method had recoveries of 95.4%-104.2% with relative standard deviations (RSD) of 2.32%. 3) The B[a]P in 2R4F reference cigarette and the 14 cigarette samples determined by the new method basically agreed with those determined by the GB/T 21130—2007 method. This method is simple, sensitive, reliable and suitable for detecting B[a]P in mainstream cigarette smoke.

Soxhlet extractionGas chromatography-mass spectrometryCigaretteMainstream cigarette smokeBenzo[a]pyrene(B[a]P)

申钦鹏、刘春波、张凤梅、何沛、司晓喜、荆磊磊、王晋、缪明明、刘志华、杨光宇

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云南中烟工业有限责任公司技术中心 云南省烟草化学重点实验室，昆明市五华区红锦路367号 650231

昆明理工大学化学工程学院，昆明市呈贡区景明南路727号 650500

索氏提取 气相色谱-质谱法(GC/MS) 卷烟 主流烟气 苯并a芘(BaP)

国家自然科学基金云南省科学技术厅科技项目云南中烟工业有限责任公司技术中心项目

213650262014FD078JSZX2014JC04

2016

10.16135/j.issn1002-0861.20160509

烟草科技
中国烟草总公司郑州烟草研究院

烟草科技

CSTPCDCSCD北大核心EI
影响因子：1.049
ISSN：1002-0861
年,卷(期)：2016.49(5)
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