Effects of foliar spraying with selenium fertilizer on selenium content and aroma substances in flue-cured tobacco leaves
To investigate the effects of trace element selenium on aroma enhancement of tobacco leaves,foliar spraying experiments with selenium fertilizers at different concentrations,0(CK),5.25 g·ha-1(S0),10.50 g·ha-1(S1),15.75 g·ha-1(S2),21.00 g·ha-1(S3)and 26.25 g·ha-1(S4),and the effects of selenium fertilizers on selenium content(mass fraction),petroleum ether extracts and volatile aroma substances in cured leaves were studied.The results showed that:1)Selenium content in tobacco leaves of the control treatment was only about 0.15 mg·kg-1,and the selenium content was significantly increased by the selenium fertilizer with the highest content at about 2.49 mg·kg-1.2)With increasing selenium fertilizer concentration,the petroleum ether extract content first increased and then decreased,reaching the highest level in leaves of S3 treatment.The petroleum ether extract content in the upper and middle leaves of S3 treatment increased by 25.71%and 44.56%,respectively compared to that of the control.3)After the application of the selenium fertilizer,the total amount of aroma substances in tobacco leaves increased significantly,with S1 treatment being the highest for upper leaves and S2 being the highest for middle leaves,which increased by 25.88%and 10.82%,respectively compared to that of the control.4)The VIP value obtained by PLS-DA was used to screen for differential markers of aroma substances in tobacco leaves from different stalk positions.Combined with cluster analysis,15 common aroma differential markers were found in the upper and middle leaves.KEGG pathway enrichment analysis indicated that selenium was involved in the regulation of terpenoid synthesis and phenylpropanoid metabolism to enhance the tobacco aroma substances.5)Correlation analysis results showed that selenium content in tobacco leaves was significantly positively correlated with petroleum ether extract and phenylalanine degradation products.
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