To identify the pathogenic fungi responsible for mildew in fermented cigar tobacco leaves from Chenzhou City,Hunan Province,48 fungal strains were isolated and purified by tissue culture method,among which Aspergillus sp.(20.83%)and Penicillium sp.(20.83%)accounted for the highest proportion.The cultivable fungi in the fermented cigar tobacco leaves were identified by morphological and molecular biological methods,and the mildew-causing ability of 12 representative fungi was determined according to Koch's postulate.Strain CX15,identified as Penicillium citrinum,and strain CX35 as Aspergillus insulicola,were found to have strong mildew-causing ability on cigar tobacco.The results of the culture temperature and relative humidity tests showed that strains CX15 and CX35 could grow in the temperature range between 25-40℃,and the optimal growth temperature was 30℃.They could grow in the relative humidity range of 50%-90%.When the relative humidity was 90%,strain CX15 grew the fastest,and when the relative humidity was 80%,strain CX35 grew the fastest.
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