湖南郴州发酵雪茄烟叶可培养真菌的分离鉴定及致霉性分析
Isolation,identification,and mildew-causing ability of cultivable fungi in fermented cigar tobacco from Chenzhou City,Hunan Province
柏心怡 1朱俊子 1刘璐 2何斌 2余金龙 2李宏光 2黄德文 2钟杰 1肖艳松2
作者信息
- 1. 湖南农业大学植物保护学院,长沙市芙蓉区农大路1号 410128
- 2. 湖南省烟草公司郴州市公司,湖南省郴州市北湖区燕泉街道燕泉北路61号 423000
- 折叠
摘要
为明确湖南省郴州产区发酵雪茄烟叶霉变的病原菌,采用组织分离法分离、纯化得到48株真菌.其中,占比最高的为曲霉属(Aspergillus sp.,20.83%)和青霉属(Penicillium sp.,20.83%).运用形态学和分子生物学相结合的方法对发酵雪茄烟叶可培养真菌进行了鉴定,并依据柯赫氏法则对12种代表真菌进行致霉性测定,结果发现鉴定为桔青霉(Penicillium citrinum)的菌株CX15和胰岛曲霉(Aspergillus insulicola)的菌株CX35对雪茄烟叶具有强致霉性.培养温度和相对湿度测试结果表明,CX15和CX35菌株在温度为25～40℃范围内均能生长,菌株的最适生长温度为30℃.CX15和CX35菌株在50%～90%相对湿度范围内均能生长,当相对湿度为90%时,CX15菌株生长最快,当相对湿度为80%时,CX35菌株的生长速度最快.
Abstract
To identify the pathogenic fungi responsible for mildew in fermented cigar tobacco leaves from Chenzhou City,Hunan Province,48 fungal strains were isolated and purified by tissue culture method,among which Aspergillus sp.(20.83%)and Penicillium sp.(20.83%)accounted for the highest proportion.The cultivable fungi in the fermented cigar tobacco leaves were identified by morphological and molecular biological methods,and the mildew-causing ability of 12 representative fungi was determined according to Koch's postulate.Strain CX15,identified as Penicillium citrinum,and strain CX35 as Aspergillus insulicola,were found to have strong mildew-causing ability on cigar tobacco.The results of the culture temperature and relative humidity tests showed that strains CX15 and CX35 could grow in the temperature range between 25-40℃,and the optimal growth temperature was 30℃.They could grow in the relative humidity range of 50%-90%.When the relative humidity was 90%,strain CX15 grew the fastest,and when the relative humidity was 80%,strain CX35 grew the fastest.
关键词
湖南郴州/雪茄烟叶/发酵/致霉性Key words
Chenzhou City,Hunan Province/Cigar tobacco/Fermentation/Mildew-causing ability引用本文复制引用
出版年
2025
国家科技期刊平台
NSTL
万方数据