首页|Reconstructing cell lineage trees with genomic barcoding:approaches and applications
Reconstructing cell lineage trees with genomic barcoding:approaches and applications
扫码查看
点击上方二维码区域,可以放大扫码查看
原文链接
NETL
NSTL
维普
万方数据
In multicellular organisms,developmental history of cell divisions and functional annotation of terminal cells can be organized into a cell lineage tree(CLT).The reconstruction of the CLT has long been a major goal in developmental biology and other related fields.Recent technological advancements,especially those in editable genomic barcodes and single-cell high-throughput sequencing,have sparked a new wave of experimental methods for reconstructing CLTs.Here we review the existing experimental approaches to the reconstruction of CLT,which are broadly categorized as either image-based or DNA barcode-based methods.In addition,we present a summary of the related literature based on the biological insight pro-vided by the obtained CLTs.Moreover,we discuss the challenges that will arise as more and better CLT data become available in the near future.Genomic barcoding-based CLT reconstructions and analyses,due to their wide applicability and high scalability,offer the potential for novel biological discoveries,especially those related to general and systemic properties of the developmental process.
Zizhang Li、Wenjing Yang、Peng Wu、Yuyan Shan、Xiaoyu Zhang、Feng Chen、Junnan Yang、Jian-Rong Yang
展开 >
Advanced Medical Technology Center,The First Affiliated Hospital,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,Guangdong 510080,China
Department of Genetics and Biomedical Informatics,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,Guangdong 510080,China
Key Laboratory of Tropical Disease Control,Ministry of Education,Sun Yat-sen University,Guangzhou,Guangdong 510080,China
National Key Research and Development Program of ChinaNational Key Research and Development Program of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaScience and Technology Planning Project of Guangdong Province,China