遗传学报2024，Vol.51Issue(3) ：302-312.DOI:10.1016/j.jgg.2023.09.013

The genome-wide meiotic recombination landscape in ciliates and its implications for crossover regulation and genome evolution

Lu Fu Chen Gu Kazufumi Mochizuki Jie Xiong Wei Miao Guangying Wang
遗传学报2024，Vol.51Issue(3) ：302-312.DOI:10.1016/j.jgg.2023.09.013
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The genome-wide meiotic recombination landscape in ciliates and its implications for crossover regulation and genome evolution

Lu Fu 1Chen Gu 1Kazufumi Mochizuki 2Jie Xiong 3Wei Miao 4Guangying Wang5
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作者信息

  • 1. Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China
  • 2. Institute of Human Genetics(IGH),CNRS,University of Montpellier,34396 Montpellier,France
  • 3. Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China;Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture,Chinese Academy of Sciences,Wuhan,Hubei 430072,China
  • 4. Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China;University of Chinese Academy of Sciences,Beijing 100049,China;Key Laboratory of Lake and Watershed Science for Water Security,Chinese Academy of Sciences,Nanjing,Jiangsu 210008,China
  • 5. Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China
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Abstract

Meiotic recombination is essential for sexual reproduction and its regulation has been extensively studied in many taxa.However,genome-wide recombination landscape has not been reported in ciliates and it re-mains unknown how it is affected by the unique features of ciliates:the synaptonemal complex(SC)-in-dependent meiosis and the nuclear dimorphism.Here,we show the recombination landscape in the model ciliate Tetrahymena thermophila by analyzing single-nucleotide polymorphism datasets from 38 hybrid progeny.We detect 1021 crossover(CO)events(35.8 per meiosis),corresponding to an overall CO rate of 9.9 cM/Mb.However,gene conversion by non-crossover is rare(1.03 per meiosis)and not biased towards G or C alleles.Consistent with the reported roles of SC in CO interference,we find no obvious sign of CO interference.CO tends to occur within germ-soma common genomic regions and many of the 44 identified CO hotspots localize at the centromeric or subtelomeric regions.Gene ontology analyses show that CO hotspots are strongly associated with genes responding to environmental changes.We discuss these re-sults with respect to how nuclear dimorphism has potentially driven the formation of the observed recombination landscape to facilitate environmental adaptation and the sharing of machinery among meiotic and somatic recombination.

Key words

Meiotic recombination/Hotspot/Crossover interference/Adaptive evolution

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基金项目

Protist 10,000 Genomes Project(P10K)

Wuhan Branch,Supercomputing Center,Chinese Academy of Sciences,China()

National Aquatic Biological Resource Center(NABRC)()

Bureau of Frontier Sciences and Education,Chinese Academy of Sciences(ZDBS-LY-SM026)

国家自然科学基金(32370457)

国家自然科学基金(32122015)

国家自然科学基金(32130011)

国家自然科学基金(31900316)

国家自然科学基金(31900339)

中国科学院战略规划重点项目(XDB0480000)

PJA3 grant of ARC Foundation(ARCPJA2021060003830)

Equipes 2022 grant of Foundation Recherche Médicale(EQU202203014651)

出版年

2024
遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCD
影响因子：0.821
ISSN：1673-8527
参考文献量75
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