首页|The genome-wide meiotic recombination landscape in ciliates and its implications for crossover regulation and genome evolution

The genome-wide meiotic recombination landscape in ciliates and its implications for crossover regulation and genome evolution

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Meiotic recombination is essential for sexual reproduction and its regulation has been extensively studied in many taxa.However,genome-wide recombination landscape has not been reported in ciliates and it re-mains unknown how it is affected by the unique features of ciliates:the synaptonemal complex(SC)-in-dependent meiosis and the nuclear dimorphism.Here,we show the recombination landscape in the model ciliate Tetrahymena thermophila by analyzing single-nucleotide polymorphism datasets from 38 hybrid progeny.We detect 1021 crossover(CO)events(35.8 per meiosis),corresponding to an overall CO rate of 9.9 cM/Mb.However,gene conversion by non-crossover is rare(1.03 per meiosis)and not biased towards G or C alleles.Consistent with the reported roles of SC in CO interference,we find no obvious sign of CO interference.CO tends to occur within germ-soma common genomic regions and many of the 44 identified CO hotspots localize at the centromeric or subtelomeric regions.Gene ontology analyses show that CO hotspots are strongly associated with genes responding to environmental changes.We discuss these re-sults with respect to how nuclear dimorphism has potentially driven the formation of the observed recombination landscape to facilitate environmental adaptation and the sharing of machinery among meiotic and somatic recombination.

Meiotic recombinationHotspotCrossover interferenceAdaptive evolution

Lu Fu、Chen Gu、Kazufumi Mochizuki、Jie Xiong、Wei Miao、Guangying Wang

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Key Laboratory of Aquatic Biodiversity and Conservation,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan,Hubei 430072,China

University of Chinese Academy of Sciences,Beijing 100049,China

Institute of Human Genetics(IGH),CNRS,University of Montpellier,34396 Montpellier,France

Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture,Chinese Academy of Sciences,Wuhan,Hubei 430072,China

Key Laboratory of Lake and Watershed Science for Water Security,Chinese Academy of Sciences,Nanjing,Jiangsu 210008,China

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Protist 10,000 Genomes ProjectWuhan Branch,Supercomputing Center,Chinese Academy of Sciences,ChinaNational Aquatic Biological Resource Center(NABRC)Bureau of Frontier Sciences and Education,Chinese Academy of Sciences国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金国家自然科学基金中国科学院战略规划重点项目PJA3 grant of ARC FoundationEquipes 2022 grant of Foundation Recherche Médicale

P10KZDBS-LY-SM0263237045732122015321300113190031631900339XDB0480000ARCPJA2021060003830EQU202203014651

2024

10.1016/j.jgg.2023.09.013

遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCD
影响因子：0.821
ISSN：1673-8527
年,卷(期)：2024.51(3)
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