HvMPK4 phosphorylates HvWRKY1 to enhance its suppression of barley immunity to powdery mildew fungus

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外文摘要：Mitogen-activated protein kinase(MAPK)cascades play important roles in disease resistance in model plant species.However,the functions of MAPK signaling pathways in crop disease resistance are largely unknown.Here we report the function of HvMKK1-HvMPK4-HvWRKY1 module in barley immune system.HvMPK4 is identified to play a negative role in barley immune response against Bgh,as virus-induced gene silencing of HvMPK4 results in enhanced disease resistance whilst stably overexpressing HvMPK4 leads to super-susceptibility to Bgh infection.Furthermore,the barley MAPK kinase HvMKK1 is found to specifically interact with HvMPK4,and the activated HvMKK1DD variant specifically phosphorylates HvMPK4 in vitro.Moreover,the transcription factor HvWRKY1 is identified to be a downstream target of HvMPK4 and phosphorylated by HvMPK4 in vitro in the presence of HvMKK1DD.Phosphorylation assay coupled with mutagenesis analyses identifies S122,T284,and S347 in HvWRKY1 as the major residues phosphorylated by HvMPK4.HvWRKY1 is phosphorylated in barley at the early stages of Bgh infection,which enhances its suppression on barley immunity likely due to enhanced DNA-binding and transcriptional repression activity.Our data suggest that the HvMKK1-HvMPK4 kinase pair acts upstream of HvWRKY1 to negatively regulate barley immunity against powdery mildew.

外文关键词：

BarleyMPK4WRKY1Phosphorylation Disease resistancePowdery mildew

作者：

Pengya Xue、Ling Zhang、Renchun Fan、Yanan Li、Xinyun Han、Ting Qi、Lifang Zhao、Deshui Yu、Qian-Hua Shen

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作者单位：

State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing 100101,China

CAS Center for Excellence in Biotic Interactions,College of Advanced Agricultural Sciences,University of Chinese Academy of Sciences,Beijing 100049,China

Department of Nutritional Sciences & Toxicology,University of California,Berkeley,CA 94720,USA

Lushan Botanical Garden,Chinese Academy of Science,Jiu-jiang,Jiangxi 332900,China

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基金：

国家重点研发计划国家重点研发计划中国科学院战略规划重点项目National Program on Research and Development of Transgenic Plants

项目编号：

2018YFD10007032018YFD1000700XDPB162016ZX08009-003-001

出版年：

2024

DOI：

10.1016/j.jgg.2023.05.005

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