Abstract
Autistic spectrum disorder(ASD)is a male-biased,heterogeneous neurodevelopmental disorder that af-fects approximately 1%-2%of the population.Prenatal exposure to valproic acid(VPA)is a recognized risk factor for ASD,but the cellular and molecular basis of VPA-induced ASD at the single-cell resolution is unclear.Here,we aim to compare the cellular and molecular differences in the hippocampus between male and female prenatal mice with ASD at the single-cell transcriptomic level.The transcriptomes of more than 45,000 cells are assigned to 12 major cell types,including neurons,glial cells,vascular cells,and immune cells.Cell type-specific genes with altered expression after prenatal VPA exposure are analyzed,and the largest number of differentially expressed genes(DEGs)are found in neurons,choroid plexus epithelial cells,and microglia.In microglia,several pathways related to inflammation are found in both males and females,including the tumor necrosis factor(TNF),nuclear factor kappa B(NF-KB),toll-like receptor(TLR),and mitogen-activated protein kinase(MAPK)signaling pathways,which are important for the induction of autistic-like behavior.Additionally,we note that several X-linked genes,including Bex1,Bex3,and Gria3,were among the male-specific DEGs of neurons.This pioneering study describes the landscape of the transcriptome in the hippocampus of autistic mice.The elucidation of sexual differences could provide innovative strategies for the prevention and treatment of ASD.
基金项目
国家自然科学基金(82074162)
国家自然科学基金(82274344)
Project for Capacity Promotion of Putuo District Clinical Special Disease"Stroke"()
science and Technology Innovation Project of Putuo District Health System(ptkwws201902)
science and Technology Innovation Project of Putuo District Health System(ptkwws202301)
Training Plan of"100 professionals"of Shanghai Putuo District Central Hospital(2022-RCJC-05)
Project of"XingLin Scholars Training"of Chengdu University of Traditional Chinese Medicine(YYZX2022170)
Shanghai Putuo District Health System Clinical Characteristic Special Disease Construction Project(2023tszb04)
NETL
NSTL
万方数据