Abstract
The human gut microbiome,a complex ecosystem,significantly influences host health,impacting crucial aspects such as metabolism and immunity.To enhance our comprehension and control of the molecular mechanisms orchestrating the intricate interplay between gut commensal bacteria and human health,the exploration of genome engineering for gut microbes is a promising frontier.Nevertheless,the complexities and diversities inherent in the gut microbiome pose substantial challenges to the development of effective genome engineering tools for human gut microbes.In this comprehensive review,we provide an overview of the current progress and challenges in genome engineering of human gut commensal bacteria,whether executed in vitro or in situ.A specific focus is directed towards the advancements and prospects in cargo DNA delivery and high-throughput techniques.Additionally,we elucidate the immense potential of genome engineering methods to enhance our understanding of the human gut microbiome and engineer the mi-croorganisms to enhance human health.
基金项目
国家重点研发计划(2019YFA0906700)
Guangdong Basic and Applied Basic Research Foundation(2020A1515110184)
Dr.Neher's Biophysics Laboratory for Innovative Drug Discovery(001/2020/ALC)
regular grants from Macao Science and Technology Development Fund(0056/2020/AMJ & 0063/2022/A2)
Young Qihuang Scholar funded by National Administration of Traditional Chinese Medicine(2020)()
Startup Research Grant of University of Macau(SRG2022-00020-FHS)
Faculty of Health Sciences,University of Macau()
NETL
NSTL
万方数据