Abstract
The highly conserved CLV-WUS negative feedback pathway plays a decisive role in regulating stem cell maintenance in shoot and floral meristems in higher plants,including Arabidopsis,rice,maize,and tomato.Here,we find significant natural variations in the OsCLV2c,OsCLV2d,and OsCRN1 loci in a genome-wide association study of grain shape in rice.OsCLV2a,OsCLV2c,OsCLV2d,and OsCRN1 negatively regulate grain length-width ratio and show distinctive geographical distribution,indica-japonica differentiation,and artificial selection signatures.Notably,OsCLV2a and OsCRN1 interact biochemically and genetically,suggesting that the two components function in a complex to regulate grain shape of rice.Furthermore,the genetic contributions of the haplotypes combining OsCLV2a,OsCLV2c,and OsCRN1 are significantly higher than those of each single gene alone in controlling key yield traits.These findings identify two groups of receptor-like kinases that may function as distinct co-receptors to control grain size in rice,thereby revealing a previously unrecognized role of the CLV class genes in regulating seed development and proposing a framework to understand the molecular mechanisms of the CLV-WUS pathway in rice and other crops.
基金项目
STI 2030-Major Projects(2023ZD0406902)
National Key Research and Development Program of China(2022YFD1200103)
National Key Research and Development Program of China(2023ZD04073)
National Natural Science Foundation of China(U22A20470)
National Natural Science Foundation of China(32072042)
National Natural Science Foundation of China(31821005)
Hubei Hongshan Laboratory(2022hszd025)
Hubei Hongshan Laboratory(2021hszd005)
Key Research and Development Program of Hubei(2023BBB135)
Key Research and Development Program of Hubei(2022BBA0033)
Fundamental Research Funds for the Central Universities(2662023PY002)
NETL
NSTL
万方数据