首页|Constructing the metabolic network of wheat kernels based on structure-guided chemical modification and multi-omics data

Constructing the metabolic network of wheat kernels based on structure-guided chemical modification and multi-omics data

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Metabolic network construction plays a pivotal role in unraveling the regulatory mechanism of biological ac-tivities,although it often proves to be challenging and labor-intensive,particularly with non-model organisms.In this study,we develop a computational approach that employs reaction models based on the structure-guided chemical modification and related compounds to construct a metabolic network in wheat.This construction results in a comprehensive structure-guided network,including 625 identified metabolites and additional 333 putative reactions compared with the Kyoto Encyclopedia of Genes and Genomes database.Using a com-bination of gene annotation,reaction classification,structure similarity,and correlations from transcriptome and metabolome analysis,a total of 229 potential genes related to these reactions are identified within this network.To validate the network,the functionality of a hydroxycinnamoyltransferase(TraesCS3D01G314900)for the synthesis of polyphenols and a rhamnosyltransferase(TraesCS2D01G078700)for the modification of flavonoids are verified through in vitro enzymatic studies and wheat mutant tests,respectively.Our research thus supports the utility of structure-guided chemical modification as an effective tool in identifying causal candidate genes for constructing metabolic networks and further in metabolomic genetic studies.

Metabolic networkChemical modificationGenetic studyWheat kernelMulti-omics

Zhitao Tian、Jingqi Jia、Bo Yin、Wei Chen

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National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan,Hubei 430070,China

Hubei Hongshan Laboratory,Wuhan,Hubei 430070,China

highperformance computing platformmass spectrometry platform at the National Key Laboratory of.Crop Genetic Improvement at Huazhong Agricultural UniversityYoung Topnotch Talent Cultivation Program of Hubei ProvinceNatural Science Foundation for Distinguished Young Scientists of Hubei ProvinceFirst-Class Discipline Construction Funds of College of Plant Science and Technology,Huazhong Agricultural University

2021CFA0582023ZKPY005

2024

10.1016/j.jgg.2024.02.008

遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCD
影响因子：0.821
ISSN：1673-8527
年,卷(期)：2024.51(7)