遗传学报2024，Vol.51Issue(7) ：735-748.DOI:10.1016/j.jgg.2024.02.009

The characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts

Tongyu Zhang Yingxi Zhu Xiaochen Wang Danyang Chong Haiquan Wang Dandan Bu Mengfei Zhao Lei Fang Chaojun Li
遗传学报2024，Vol.51Issue(7) ：735-748.DOI:10.1016/j.jgg.2024.02.009
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The characterization of protein lactylation in relation to cardiac metabolic reprogramming in neonatal mouse hearts

Tongyu Zhang 1Yingxi Zhu 2Xiaochen Wang 1Danyang Chong 3Haiquan Wang 1Dandan Bu 1Mengfei Zhao 1Lei Fang 1Chaojun Li3
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作者信息

  • 1. Ministry of Education Key Laboratory of Model Animals for Disease Study,Model Animal Research Center,Medical School of Nanjing University,National Resource Center for Mutant Mice,Nanjing,Jiangsu 210093,China
  • 2. State Key Laboratory of Reproductive Medicine and Offspring Health,China International Joint Research Center on Environment and Human Health,Center for Global Health,School of Public Health,Gusu School,Nanjing Medical University,Nanjing,Jiangsu 211166,China
  • 3. Ministry of Education Key Laboratory of Model Animals for Disease Study,Model Animal Research Center,Medical School of Nanjing University,National Resource Center for Mutant Mice,Nanjing,Jiangsu 210093,China;State Key Laboratory of Reproductive Medicine and Offspring Health,China International Joint Research Center on Environment and Human Health,Center for Global Health,School of Public Health,Gusu School,Nanjing Medical University,Nanjing,Jiangsu 211166,China
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Abstract

In mammals,the neonatal heart can regenerate upon injury within a short time after birth,while adults lose this ability.Metabolic reprogramming has been demonstrated to be critical for cardiomyocyte proliferation in the neonatal heart.Here,we reveal that cardiac metabolic reprogramming could be regulated by altering global protein lactylation.By performing 4D label-free proteomics and lysine lactylation(Kla)omics analyses in mouse hearts at postnatal days 1,5,and 7,2297 Kla sites from 980 proteins are identified,among which 1262 Kla sites from 409 proteins are quantified.Functional clustering analysis reveals that the proteins with altered Kla sites are mainly involved in metabolic processes.The expression and Kla levels of proteins in glycolysis show a positive correlation while a negative correlation in fatty acid oxidation.Furthermore,we verify the Kla levels of several differentially modified proteins,including ACAT1,ACADL,ACADVL,PFKM,PKM,and NPM1.Overall,our study reports a comprehensive Kla map in the neonatal mouse heart,which will help to understand the regulatory network of metabolic reprogramming and cardiac regeneration.

Key words

Lactylation/Metabolic reprogramming/Postnatal heart regeneration/Cell proliferation

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出版年

2024
遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCD
影响因子：0.821
ISSN：1673-8527
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