Abstract
Vascular plants have evolved intricate long-distance signaling mechanisms to cope with environmental stress,with reactive oxygen species(ROS)emerging as pivotal systemic signals in plant stress responses.However,the exact role of ROS as root-to-shoot signals in the drought response has not been determined.In this study,we reveal that compared with wild-type plants,ferric reductase defective 3(frd3)mutants exhibit enhanced drought resistance concomitant with elevated NINE-CIS-EPOXYCAROTENOID DIOXY-GENASE 3(NCED3)transcript levels and abscisic acid(ABA)contents in leaves as well as increased hydrogen peroxide(H2O2)levels in roots and leaves.Grafting experiments distinctly illustrate that drought resistance can be conferred by the frd3 rootstock regardless of the scion genotype,indicating that long-distance signals originating from frd3 roots promote an increase in ABA levels in leaves.Intriguingly,the drought resistance conferred by the frd3 mutant rootstock is weakened by the CAT2-overexpressing scion,suggesting that H2O2 may be involved in long-distance signaling.Moreover,the results of comparative transcriptome and proteome analyses support the drought resistance phenotype of the frd3 mutant.Taken together,our findings substantiate the notion that frd3 root-derived long-distance signals trigger ABA synthesis in leaves and enhance drought resistance,providing new evidence for root-to-shoot long-dis-tance signaling in the drought response of plants.
基金项目
National Natural Science Foundation of China(31900230)
China Postdoctoral Science Foundation(2020T130634)
China Postdoctoral Science Foundation(2019M652200)
ABRC for the FRD3 mutant seeds(frd3-8)
ABRC for the FRD3 mutant seeds(man1-1)
NETL
NSTL
万方数据