Abstract
MicroRNAs(miRNAs)play a significant role in axon regeneration following spinal cord injury.However,the functions of numerous miRNAs in axon regeneration within the central nervous system(CNS)remain largely unexplored.Here,we elucidate the positive role of microRNA-2184(miR-2184)in axon regeneration within zebrafish Mauthner cells(M-cells).The upregulation of miR-2184 in a single M-cell can facilitate axon regeneration,while the specific sponge-induced silencing of miR-2184 leads to impeded regeneration.We show that syt3,a downstream target of miR-2184,negatively regulates axon regeneration,and the regeneration suppression modulated by syt3 depends on its binding to Ca2+.Furthermore,pharmaco-logical stimulation of the cAMP/PKA pathway suggests that changes in the readily releasable pool may affect axon regeneration.Our data indicate that miR-2184 promotes axon regeneration of M-cells within the CNS by modulating the downstream target syt3,providing valuable insights into potential therapeutic strategies.
维普
万方数据