首页|Ca2+-calpains axis regulates Yki stability and activity in Drosophila

Ca2+-calpains axis regulates Yki stability and activity in Drosophila

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Yorkie(Yki)is a key effector of the Hippo pathway that activates the expression of targets by associating with the transcription factor Scalloped.Various upstream signals,such as cell polarity and mechanical cues,control transcriptional programs by regulating Yki activity.Searching for Yki regulatory factors has far-reaching significance for studying the Hippo pathway in development and human diseases.In this study,we identify Calpain-A(CalpA)and Calpain-B(CalpB),two calcium(Ca2+)-dependent modulatory proteases of the calpain family,as critical regulators of Yki in Drosophila that interact with Yki,respectively.Ca2+induces Yki cleavage in a CalpA/CalpB-dependent manner,and the protease activity of CalpA/CalpB is pivotal for the cleavage.Furthermore,overexpression of CalpA or CalpB in Drosophila partially restores the large wing phenotype caused by Yki overexpression,and F98 of Yki is an important cleavage site by the Ca2+-calpains axis.Our study uncovers a unique mechanism whereby the Ca2+-calpain axis modulates Yki activity through protein cleavage.

CalpACalpBCalciumHippo pathwayYkiDrosophila

Chaojun Zhai、Yunfeng Wang、Shenao Qi、Muhan Yang、Shian Wu

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The State Key Laboratory of Medicinal Chemical Biology,Tianjin Key Laboratory of Protein Sciences,College of Life Sciences,Nankai University,Tianjin 300071,China

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaFundamental Research Funds for the Central Universities,Nankai University

32170714319707333167151363185024

2024

遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCD
影响因子:0.821
ISSN:1673-8527
年,卷(期):2024.51(10)