遗传学报2024，Vol.51Issue(10) ：1101-1110.DOI:10.1016/j.jgg.2024.05.011

Genome-editing of a circadian clock gene TaPRR95 facilitates wheat peduncle growth and heading date

Mingxue Fu Shaoshuai Liu Yuqing Che Dada Cui Zhongyin Deng Yang Li Xinyu Zou Xingchen Kong Guoliang Chen Min Zhang Yifan Liu Xiang Wang Wei Liu Danmei Liu Shuaifeng Geng Aili Li Long Mao
遗传学报2024，Vol.51Issue(10) ：1101-1110.DOI:10.1016/j.jgg.2024.05.011
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  • 维普
  • 万方数据

Genome-editing of a circadian clock gene TaPRR95 facilitates wheat peduncle growth and heading date

Mingxue Fu 1Shaoshuai Liu 1Yuqing Che 1Dada Cui 1Zhongyin Deng 1Yang Li 1Xinyu Zou 1Xingchen Kong 2Guoliang Chen 3Min Zhang 3Yifan Liu 4Xiang Wang 4Wei Liu 5Danmei Liu 5Shuaifeng Geng 1Aili Li 1Long Mao1
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作者信息

  • 1. State Key Laboratory of Crop Gene Resources and Breeding and National Key Facility for Crop Resources and Genetic Improvement,Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • 2. College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007,China
  • 3. College of Agronomy,Sichuan Agricultural University,Chengdu,Sichuan 610106,China
  • 4. College of Agronomy,Henan Agricultural University,Zhengzhou,Henan 450002,China
  • 5. School of Life Science,Shanxi University,Taiyuan,Shanxi 030006,China
  • 折叠

Abstract

Plant height and heading date are important agronomic traits in wheat(Triticum aestivum L.)that affect final grain yield.In wheat,knowledge of pseudo-response regulator(PRR)genes on agronomic traits is limited.Here,we identify a wheat TaPRR95 gene by genome-wide association studies to be associated with plant height.Triple allele mutant plants produced by CRISPR/Cas9 show increased plant height,particularly the peduncle,with an earlier heading date.The longer peduncle is mainly caused by the increased cell elon-gation at its upper section,whilst the early heading date is accompanied by elevated expression of flow-ering genes,such as TaFT and TaCO1.A peduncle-specific transcriptome analysis reveals up-regulated photosynthesis genes and down-regulated IAA/Aux genes for auxin signaling in prr95aabbdd plants that may act as a regulatory mechanism to promote robust plant growth.A haplotype analysis identifies a TaPRR95-B haplotype(Hap2)to be closely associated with reduced plant height and increased thousand-grain weight.Moreover,the Hap2 frequency is higher in cultivars than that in landraces,suggesting the artifi-cial selection on the allele during wheat breeding.These findings suggest that TaPRR95 is a regulator for plant height and heading date,thereby providing an important target for wheat yield improvement.

Key words

Pseudo-response regulator(PRR)/TaPRR95/CRISPR/Cas9/Plant height/Heading date/Photosynthesis/Phytohormone/Wheat

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基金项目

STI 2030-Major Projects(2023ZD0406802)

National Natural Science Foundation of China(32072066)

National Natural Science Foundation of China(32172050)

National Natural Science Foundation of China(3220151460)

Hainan Yazhou Bay Seed Lab(B21HJ0215)

CAAS Agricultural Science and Technology Innovation Program(CAAS-ZDRW202002)

CAAS Agricultural Science and Technology Innovation Program(CAAS-ZDRW202201)

Hebei Natural Science Foundation(C2021205013)

The"Yellow River Delta Scholar"in Sino-Agro Experimental Station for Salt Tolerant Crops(SAESSTC)()

出版年

2024
遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCDCSCD
影响因子：0.821
ISSN：1673-8527
参考文献量39
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