首页|A single-nucleotide polymorphism in PvPW1 encodingβ-1,3-glucanase 9 is associated with pod width in Phaseolus vulgaris L.

A single-nucleotide polymorphism in PvPW1 encodingβ-1,3-glucanase 9 is associated with pod width in Phaseolus vulgaris L.

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Pod width influences pod size,shape,yield,and consumer preference in snap beans(Phaseolus vulgaris L.).In this study,we map PvPW1,a quantitative trait locus associated with pod width in snap beans,through genotyping and phenotyping of recombinant plants.We identify Phvul.006G072800,encoding theβ-1,3-glucanase 9 protein,as the causal gene for PvPW1.The PvPW1G3555 allele is found to positively regulate pod width,as revealed by an association analysis between pod width phenotype and the PvPW1G3555C genotype across 17 bi-parental F2 populations.In total,97.7％of the 133 wide pod acces-sions carry PvPW1G3555 while 82.1％of the 78 narrow pod accessions carry PvPW1C3555,indicating strong selection pressure on PvPW1 during common bean breeding.Re-sequencing data from 59 common bean cultivars identify an 8-bp deletion in the intron linked to PvPW1C3555,leading to the development of the InDel marker of PvM436.Genotyping 317 common bean accessions with PvM436 demonstrated that accessions with PvM436247 and PvM436227 alleles have wider pods compared to those with PvM436219 allele,establishing PvM436 as a reliable marker for molecular breeding in snap beans.These findings highlight PvPW1 as a critical gene regulating pod width and underscore the utility of PvM436 in marker-assisted selection for snap bean breeding.

Common beanPod widthFine-mappingβ-1,3-glucanase 9Marker-assisted selection

Kun Xu、Jinlong Zhu、Hong Zhai、Qiang Yang、Keqin Zhou、Qijian Song、Jing Wu、Dajun Liu、Yanhua Li、Zhengjun Xia

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State Key Laboratory of Black Soils Conservation and Utilization,Key Laboratory of Soybean Molecular Design Breeding,Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences,Harbin,Heilongjiang 150081,China

USDA ARS,Soybean Genome & Improvement Lab,Beltsville 20705,USA

Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 10081,China

Horticulture Department,College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin,Heilongjiang 150000,China

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2024

10.1016/j.jgg.2024.09.020

遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCD
影响因子：0.821
ISSN：1673-8527
年,卷(期)：2024.51(12)