首页|Synchronized lineage tracing of cell membranes and nuclei by dual recombinases and dual fluorescent

Synchronized lineage tracing of cell membranes and nuclei by dual recombinases and dual fluorescent

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Genetic lineage tracing has been widely employed to investigate cell lineages and fate.However,con-ventional reporting systems often label the entire cytoplasm,making it challenging to discern cell bound-aries.Additionally,single Cre-loxP recombination systems have limitations in tracing specific cell populations.This study proposes three reporting systems utilizing Cre,Dre,and Dre+Cre mediated recombination.These systems incorporate tdTomato expression on the cell membrane and PhiYFP expression within the nucleus,allowing for clear observation of the nucleus and membrane.The efficacy of these systems is successfully demonstrated by labeling cardiomyocytes and hepatocytes.The potential for dynamic visualization of the cell membrane is showcased using intravital imaging microscopy or three-dimensional imaging.Furthermore,by combining this dual recombinase system with the ProTracer sys-tem,hepatocyte proliferation is traced with enhanced precision.This reporting system holds significant importance for advancing the understanding of cell fate studies in development,homeostasis,and diseases.

Lineage tracingDual recombinases systemCre-IoxPDre-roxCell membrane and nucleus labelingProTracer system

Xueying Yang、Shun He、Xufeng Li、Zhihou Guo、Haichang Wang、Zhuonan Zhang、Xin Song、Ke Jia、Lingjuan He、Bin Zhou

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School of Life Science,Hangzhou Institute for Advanced Study,University of Chinese Academy of Sciences,Hangzhou,Zhejiang 310024,China

School of Life Sciences,Westlake University,Hangzhou,Zhejiang 310030,China

New Cornerstone Science Laboratory,State Key Laboratory of Cell Biology,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200031,China

School of Life Science and Technology,ShanghaiTech University,Shanghai 201210,China

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2024

10.1016/j.jgg.2024.07.006

遗传学报
中国遗传学会 中国科学院遗传与发育生物学研究所

遗传学报

CSTPCD
影响因子：0.821
ISSN：1673-8527
年,卷(期)：2024.51(12)