摘要
目的:研究小叶黄杨叶中的化学成分抑制非小细胞肺癌A549增殖作用.方法:通过回流提取、萃取、硅胶色谱柱与质谱、核磁等方法进行分离纯化及结构确证.采用CCK8法,对分离得到化合物进行非小细胞肺癌A549体外活性评价.结果:从小叶黄杨叶中分离得到8个化合物,鉴定为白桦脂醇(1)、羽扇豆醇(2)、羟基环原黄杨碱(3)、羟基环原黄杨碱酯(4)、N-甲基黄杨宁(5)、苦参素(6)、3-氨基-4-(羟基甲基)-4,14-二甲基-9,19-环丙基-17-二甲基氨基-23-苯甲酰酯甾烷(7)、3-氨基-4,14-二甲基-9,19-环丙基-16-甲基丁酸甲酯-17-二甲基氨基-23-苯甲酰酯甾烷(8).活性结果表明化合物1-8均能抑制非小细胞肺癌A549增殖.结论:化合物5、7、8为首次从小叶黄杨叶中分离得到的新化合物;苦参素(6)为首次从小叶黄杨中叶分离得到.化合物7抗非小细胞肺癌A549作用最强.
Abstract
Objective:To study the inhibitory effect of chemical components in the leaves of buxus microphylla on the proliferation of non-small cell lung cancer A549.Methods:Separation,purification,and structural confirma-tion were carried out through reflux extraction,extraction,silica gel chromatography column and mass spectrom-etry,nuclear magnetic resonance,and other methods.Using the CCK8 method,evaluate the in vitro activity of the isolated compound for non-small cell lung cancer A549.Results:Eight compounds were isolated from the leaves of small leaved boxwood and identified as betulin alcohol(1),lupin alcohol(2),hydroxycyclo boxwood alkaloid(3),hydroxy cyclo boxwood alkaloid ester(4),N-methyl boxwood tannin(5),matrine(6),3-a-mino-4-(hydroxymethyl)-4,14-dimethyl-9,19-cyclopropyl-17-dimethylamino-23-benzoyl ester sterane(7),3-amino-4,14-dimethyl-9,19-cyclopropyl-16-methylbutyrate-methyl-17-dimethyl-amino-23-benzoyl ester sterane(8).The activity results indicate that compounds 1-8 can inhibit the prolifera-tion of non-small cell lung cancer A549.Conclusion:Compounds 5,7,and 8 are new compounds isolated from the small leaved yellow poplar for the first time.Oxymatrine(6)was isolated from Buxus Microphylla for the first time.Compound 7 has the strongest anti non-small cell lung cancer effect on A549.
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