Extraction Method and Process Optimization of Jiangxi Fructus Aurantii Pectin
In order to investigate the extraction rate of pectin from Jiangxi Fructus Aurantii by water extraction and alcohol precipitation method and acid extraction and alcohol precipitation method,and to explore the technological conditions of extracting pectin from Jiangxi Fructus Aurantii by acid extraction and alcohol precipitation method,two extraction methods were used to extract pectin from Jiangxi Fructus Aurantii,and the extraction rate was cal-culated.The single factor experiment was carried out for the acid extraction and alcohol precipitation method.Based on the results of single factor experiment,and the optimum extraction process of pectin polysaccharide from Jian-gxi Fructus Aurantii was determined by Box-Behnken response surface experimental design scheme.The results showed that the extraction rate of polysaccharide extracted by water extraction and alcohol precipitation was 0.92％,and the extraction rate of polysaccharide extracted by acid extraction and alcohol precipitation was 17.18％.The optimum extraction process of pectin polysaccharide from Jiangxi Fructus Aurantii by acid extraction and alcohol precipitation was as follows:liquid-solid ratio 22:1(mL/g),pH0.75,extraction time 100 min,extraction temperature 81 ℃,the theoretical extraction rate was 23.34％.According to this extraction condition,three parallel experiments were carried out,and the extraction rate of pectin polysaccharide from Jiangxi Fructus Aurantii was 23.28±0.51％,which was consistent with the predicted value.The results showed that the extrac-tion rate of acid extraction and alcohol precipitation method was higher than that of water extraction and alcohol precipitation method.The optimization of extraction process of Jiangxi Fructus Aurantii through response surface methodology is feasible,with simple operation and improved pectin extraction rate.
万方数据
维普
