EFFECT OF HYPERHOMOCYSTEINEMIA ON BLOOD GLUCOSE HOMEOSTASIS
Objective To explore the effects of hyperhomocysteinemia(HHcy)on blood glucose homeostasis.Methods C57BL/6 mice were randomly divided into two groups:control group(CHOW)and model group(HMD),10 mice per group.The CHOW group was fed the AIN-93G diet,and the HMD group fed the AIN-93G+2%methionine diet.The oral glucose tolerance test(OGTT),insulin tolerance test(ITT),and insulin secretion experiment were performed.The morphological changes in the pancreas were observed by hematoxylin-eosin(HE)staining,pancreatic insulin expression by immunofluorescence staining,and total pancreatic insulin content by enzyme-linked immunosorbent assay(ELISA).Results Compared with the CHOW group,after 1 month of feeding,plasma Hcy was significantly increased in the HMD group(P<0.0001).After 4 months of feeding,there was no significant difference in fasting blood glucose between the two groups,but mice in the HMD group had significantly increased random blood glucose(P<0.0001),impaired glucose tolerance(P<0.001)and insulin tolerance(P<0.001);Compared with the CHOW group,the glucose-stimulated insulin secretion was reduced in the HMD group(P<0.0001).The results of HE staining showed that there was no significant change in the pancreatic morphology between the two groups.Immunofluorescence chemical analysis revealed enhanced insulin fluorescence intensity in the pancreas of the HMD group(P<0.001).Compared with the CHOW group,the total pancreatic insulin content increased significantly in the HMD group.The difference was statistically significant(P<0.0001).Conclusion HHcy can cause the development of type 2 diabetes mellitus(T2DM),and homocysteine may affect blood glucose homeostasis by damaging insulin secretion function.[ACTA NUTRIMENTA SINICA,2024,46(1):22-27]
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