EFFECTS OF VITAMIN C INTERVENTION ON IMMUNE FUNCTION IN HIGH TEMPERATURE-EXPOSED RATS
Objective To investigate the effects of vitamin C(VC)intervention on immune function in rats exposed to high-temperature.Methods Twenty-four SPF 8-week-old Sprague-Dawley(SD)rats were randomly divided into four groups:normal temperature control(NC),high temperature exposure(HE),HE supplemented with 10 mg/kg VC and 14 mg/kg VC.Two intervention groups were administrated intragastrically with 1.0 ml VC solution at the concentration of 1.0 g/L or 1.4 g/L,and the other groups were given the same amount of distilled water every morning.The rats ran on a treadmill in a normal environment(22±1)℃or a heat environment(33±1)℃for 30 min in the morning and in the afternoon daily,consecutive six days once a week for four weeks.The rats were sacrificed on the 29th day.Flow cytometry was used to analyze the proportions of CD3+,CD4+,CD8+,CD16+ and CD45+ cells in peripheral blood.The levels of IgA,IgG,IgM,C3,and TNF-α,IL-2,IL-6 in the serum were measured by enzyme-linked immuosorbent assay.Results Compared with the NC group,the percentages of T cells,NK cells,WBC,CD4+/CD8+ ratio in the blood,and the contents of IL-6,IgA,IgG and C3 in the serum were significantly decreased.The IL-2 level was significantly increased in the HE group(all P<0.05).Compared with the HE group,the percentages of T cells and NK cells in the 10 mg/kg VC and 14 mg/kg VC groups,as well as C D4+/CD8+ ratio,the percentage of WBC,the contents of IgA,IgG,IgM,C3,TNF-α and IL-6 in the 14 mg/kg VC group were significantly increased(P<0.05).There was no significant difference in the content of IL-2 between two VC supplementation groups and the HE group.Conclusion High temperature exposure has profound effect on both cell-mediated immunity and humoral immunity in rats.The intervention by 14 mg/kg VC can alleviate those negative effects caused by high temperature exposure.[ACTA NUTRIMENTA SINICA,2024,46(1):28-33]
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