AMELIORATING EFFECTS OF CURCUMIN ON HEPATIC STEATOSIS IN HIGH FAT DIET FED MICE
Objective To study the effects of curcumin on expressions of hepatic Nrf2/Keap1-targeted microRNAs and Nrf2/Keap1,redox status,and lipid metabolism in high fat diet fed mice.Methods Twenty-four male C57BL/6J mice were randomly divided into three groups:normal control(NC)group,high fat(HF)diet fed group,high fat and curcumin(Cur)group.The mice from the Cur group were fed a high fat diet and received curcumin treatment at the dose of 200 mg/(kg·d)by oral gavage.Twelve weeks later,hepatic histology,expressions of hepatic miRs that targeted on Nrf2/Keap1,and genes in the Nrf2 signaling pathway and lipid metabolism,as well as the oxidative stress biomarkers were determined.Results Compared with the HF group,curcumin effectively improved plasma lipid profiles and inhibited hepatic fat accumulation possibly by down-regulating miR-28,miR-27a and miR-34a and up-regulating miR-200a,resulting in enhanced Nrf2 nuclear translocation and up-regulation of NQO1 and HO-1.Additionally,hepatic GPx,SOD,and CAT activities and GSH level were significantly higher in the Cur group than those in the HF group,while MDA level was remarkably lower.Curcumin also down-regulated the expressions of lipogenesis genes PPARγ and C/EBPα,and up-regulated the expressions of fatty acid oxidation genes PPARα and CPT1α.Conclusion Curcumin can prevent high fat diet induced non-alcoholic fatty liver disease by maintaining hepatic redox status and lipid metabolism via miR/Nrf2 signaling pathway.[ACTA NUTRIMENTA SINICA,2024,46(1):48-55]
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