S-EQUOL IMPROVES SODIUM OLEATE-INDUCED STEATOSIS BY REGULATING PI3K/AKT/NF-κB SIGNALING PATHWAY THROUGH ERβ IN BRL CELLS
Objective To observe the effect of S-equol(S-Eq)on sodium oleate(NaOL)-induced steatosis in BRL cells,and to investigate its mechanism of action.Methods BRL cells were cultured in vitro,treated with different concentrations of S-Eq,NaOL and PHTPP for 48h.The appropriate concentration of NaOL for the induction of BRL cell steatosis and the appropriate concentration of S-Eq and PHTPP for intervention were determined by cell viability,oil red O staining and cell TG content.The cells were divided into control group,model group,model + low and high S-Eq concentration(10-6 mol/L,10-5 mol/L)groups,model + estradiol(E2,10-7 mol/L)group,model + S-Eq(10-5 mol/L)+ Fulvestrant(10-6 mol/L)group.After 48 h of intervention,the contents of TG,TNF-α and IL-6 were detected in cells,the expression levels of TNF-α and IL-6 mRNA were detected by qRT-PCR,and the expression levels of ERβ,PI3k,AKT,p-AKT,p-p-p65 proteins were detected by Western blotting.Results Compared with the control group,when the NaOL concentration was 0.48 mmol/L,the viability of BRL cells did not decrease significantly,the content of TG was significantly increased,and a large number of lipid droplets were accumulated in the cells,which was determined as the appropriate concentration of NaOL for inducing steatosis.At the end of the intervention,compared with the model group,S-Eq or E2 intervention could significantly reduce the contents of TG,TNF-α and IL-6(P<0.05),as well as the expression levels of TNF-α and IL-6 mRNA in cells(P<0.05).Meanwhile,the expressions of ERβ,PI3k,p-AKT were significant increased,and the expression level of p-p65 protein was significanty reduced(P<0.05).PHTPP could significantly inhibit the improvement effect of S-Eq on cell steatosis.Conclusion S-Eq could effectively improve NAOL-induced BRL cell steatosis,and part of the mechanism is related to the reduction of inflammatory response by S-Eq via regulating the PI3K/AKT/NF-κB signaling pathway through ERβ.[ACTA NUTRIMENTA SINICA,2024,46(1):62-68]
s-equolsteatosisphosphatidylinositol 3-kinase/serine-threonine protein kinasenuclear factor kappa-B
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