RESVERATROL ALLEVIATES HYPOXIC INJURY OF RAT ISLET Β CELL TUMOR CELLS
Objective To study the effects of resveratrol on hypoxia injury in rat islet beta tumor cells(INS-1).Methods The INS-1 cells were divided into three groups:control group,hypoxia group,hypoxia and resveratrol group(hypoxia+RSV).The cell proliferation at different hypoxia times(6,12,24,48 h)was detected by CCK8 kit.Resveratrol at the concentrations of 8,10,12,15 μmol/L were used for intervention for 12 hours.Oxidative stress levels were detected by reactive oxygen species kit and mitochondrial superoxide kit.Mitochondrial membrane potential and ATP levels were detected for mitochondrial function,and apoptosis was detected by flow cytometry.Insulin quantitative assay kit was used to detect insulin secretion,and quantitative real-time reverse transcription was used to detect the mRNA levels of Sirt1,Pdx1 and Glut2 genes.The corresponding protein levels were detected by Western blot.Results Compared with the normal oxygen control group,cell proliferation was decreased(P<0.05),apoptosis rate increased(P<0.01),mitochondrial function decreased(P<0.05),and insulin secretion decreased(P<0.05)in the hypoxia group.The expression levels of Sirt1,Pdx1,Glut2 genes and their corresponding proteins were also decreased(P<0.05).The intervention by resveratrol could significantly reduce hypoxia stress induced damage(P<0.05),promote proliferation(P<0.05),decrease apoptosis(P<0.01),improve mitochondrial function(P<0.05),and promote insulin secretion(P<0.05)in INS-1 cells exposed to hypoxia.The expression levels of Sirt1,Pdx1,Glut2 genes and their corresponding proteins were also improved in response to resveratrol intervention(P<0.05).Conclusion Resveratrol can protect INS-1 cells against oxidative stress caused by hypoxia,improve mitochondrial function,inhibit apoptosis,promote insulin function gene expression and increase insulin secretion.[ACTA NUTRIMENTA SINICA,2024,46(1):69-74]
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